Fig. 4

Calculation of gene-wise ITH. A Schematic presentation of the calculation processes of integrated heterogeneity score (IHS). B Spearman correlation between intra-tumor variability scores and clustering concordance scores. C Heatmap of the expression level of the top 10 genes with lowest and highest IHS. D The distribution of IHS of protein-coding genes. E Patient group overall ratio (PGOR) curves of genes with varying IHS. Lower PGOR indicates higher ITH. F Enrichment results of top 100 genes with lowest IHS of our cohort against ranked IHS results from other four public cohorts. G Enrichment map based on GSEA results. Blue nodes represent biological processes associated with low-ITH genes, while red nodes represent biological processes related to high-ITH genes. The thickness of edges is proportional to the overlap between the gene sets. Biological processes with similar function were grouped in cluster and labeled manually