Fig. 2

Whole-brain imaging of microglia and infiltrating immune cells after ischemic stroke. A Schematic representation of bone marrow transplantation. The bone marrow from Vav1-Ai9 mice was transplanted into Tmem119-Ai6 mice to obtain chimeric mice with microglia labeled with ZsGreen (green), and periphery immune cells labeled with tdTomato (red). B Microglia and infiltrating immune cells in the stroke brain. Chimeric mice were subjected to permanent stroke. On days 1 and 3 after stroke, the brains were collected for whole brain imaging. Transverse views of microglia (ZsGreen; above) and 3D whole-brain views of infiltrating immune cells (tdTomato; below) are shown here. C Microglia and infiltrating immune cells in the peri-infarct area. Enlarged images are shown below. D A chimeric mouse with periphery immune cells labeled with tdTomato (red) was subjected to permanent stroke. The brain was collected on post-stroke day 7. Associated videos are available in the supplemental materials